human liver tumor tissue microarray  (Biomax Inc)

Journal: Journal of Translational Medicine

Article Title: Ruxolitinib suppresses liver fibrosis progression and accelerates fibrosis reversal via selectively targeting Janus kinase 1/2

doi: 10.1186/s12967-022-03366-y

Figure Lengend Snippet: Up-regulated JAK1 and JAK2 are associated with liver fibrosis and liver disease in human and mice. A JAK1 and JAK2 immunohistochemistry (IHC) was performed in human liver tissues microarray of normal, cirrhosis, and cancer (× 100 magnification, scale bar = 100 μm). B JAK1 and JAK2 scores quantified by IHC in normal liver, liver cirrhosis, and liver cancer. C The IHC and Sirius red staining of JAK1 and JAK2 were performed in mouse normal and fibrosis for 6 weeks CCl 4 -induced or 8 weeks CCl 4 -induced liver tissues (× 100 and × 200 magnification, scale bar = 100 μm). D JAK1 and JAK2 scores quantified by IHC in normal liver, 6 weeks CCl 4 -induced liver fibrosis and 8 weeks CCl 4 -induced liver fibrosis. Data presented are means ± SD. ** P < 0.01; *** P < 0.001

Article Snippet: Human liver tissue microarray was purchased from Biomax, including 5 liver samples with normal liver tissue, 22 liver samples with liver cirrhosis, and 53 liver samples with liver cancer which were collected in US.

Techniques: Immunohistochemistry, Microarray, Staining

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Staining

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Western Blot